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大鼠elisa试剂盒

火马电竞 > 产品

大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒

大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒用途

本试剂盒仅供科研使用,定量检测细胞液、体液、组织、血清、血浆等标本中大鼠核因子κB受体活化因子配基(RANKL)的含量。

YUANLI:

CAIYONGJINGZHENGFACEDINGDASHUHEYINZIκBSHOUTIHUOHUAYINZIPEIJI(RANKL)SHUIPING。YONGDASHUHEYINZIκBSHOUTIHUOHUAYINZIPEIJI(RANKL)KANGYUANBAOBEIWEIKONGBAN,ZHICHENGGUXIANGZAITI,SHIYAN

时依次在微孔板中加入标本及标准品,并加入 HRP 标记的RANKL抗体,标本及标准品中的抗体和一定量的酶标抗体竞争与

GUXIANGKANGYUANJIEHE。FANFUXIDIHOUJIADIWU TMB XIANSE。TMB ZAIGUOYANGHUAWUMEIDECUIHUAXIAZHUANHUAWEILANSE,ZAIYONGLIUSUANZHONGZHIFANYING,ZHUANBIANCHENGHUANGSE。

标本中抗体量越多,结合在固相上的酶标抗体愈少,颜色越浅。颜色的深浅和样品中的RANKL含量呈负相关。采用酶标仪在

450nm 波长测定吸光度(OD 值),根据标准曲线,计算测试样品中RANKL浓度。

大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒试剂盒组成:

大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒技术流程:


火马电竞 YANGPINSHOUJI、CHULIJIBAOCUN

火马电竞 1. XIBAOPEIYANGSHANGQING:SHIYONGYUJIANCETIWAIPEIYANGDEXIBAOFENMIXINGCHENGFEN。YONGWUJUNGUANSHOUJIXIBAOSHANGQINGYE,YI1000×gLIXIN15FENZHONG,SHOUJISHANGQING。

2. XIBAO:YONGPBSFANFUXIDIXIBAO3CI,DIAOZHENGXIBAONONGDUDADAO104-106/ml ZUOYOU,TONGGUOFANFUDONGRONG,SHIXIBAOPOHUAIBINGFANGCHUXIBAONEICHENGFEN,HUO

火马电竞 ZHEXIBAOCHAOSHENGFENSUI,LIXINQUSHANGQINGYEJIANCE。

火马电竞 3. XUEQING:ZAISHIWENXIA,XUEYEZIRANNINGGU,YI1000×gLIXIN15FENZHONG,QUSHANGQINGDAICE。

火马电竞 4. XUEJIANG:YINGGENJUBIAOBENDEYAOQIUXUANZEEDTA HUONINGMENGSUANNAZUOWEIKANGNINGJI,HUNHEHOUJINGZHI10-20 FENZHONGHOU,YI1000×gLIXIN15FENZHONG,SHOUJISHANG

QING。

5. TIYE:BAOKUOXIONGFUSHUI、NAOJIYE,FENMIWUDENG。SHIYONGBUHANREYUANHENEIDUSUDELIXINGUANSHOUJI,YI1000×gLIXIN15FENZHONG,SHOUJISHANGQING。

火马电竞 6. ZUZHIBIAOBEN:QIEQUZUZHIBIAOBEN,CHENGQUZHONGLIANG0.5mg,JIARU500ulDEPBS,YONGSHOUGONGHUOYUNJIANGQI,HUOCHAOSHENGPOSUIYIJIANGBIAOBENYUNJIANG,YI2000-3000

rmpLIXIN20 FENZHONG,SHOUJISHANGQINGJINXINGJIANCE。

7. YANGPINZHONGBUNENGHANYOUNaN3,YINNaN3 YIZHILAGENGUOYANGHUAWUMEIDE(HRP)HUOXING。

8. BAOCUN:RUGUOYANGPINBUNENGLIJIJIANCE,YINGJIANGQIFENZHUANG,-70 ℃BAOCUN,BIMIANFANFULENGDONG。BAOCUNGUOCHENGZHONGRUCHUXIANCHENDIAN,YINGZAICILIXIN。XUEYEBIAO

BENJINKENENGDEBUYAOSHIYONGRONGXUEHUOGAOXUEZHIXUE。RUGUOXUEQINGZHONGHANDALIANGKELI,JIANCEQIANXIANLIXINHUOGUOLV。BUYAOZAI37℃HUOGENGGAODEWENDUJIAREJIEDONG。YINGZAISHIWENXIAJIEDONGBINGQUEBAOYANGPINJUNYUNDICHONGFENJIEDONG。


大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒操作步骤:

火马电竞 1. QUCHUSHIJIHE,SHIWEN(20-25℃)FANGZHI 30 FENZHONG。

2. FENZU:QUCHU 96 KONGBAN,GENJUDAICEYANGPINSHULIANGJIASHANGBIAOZHUNPINDESHULIANGJUEDINGSUOXUDEBANTIAOSHU,BASHENGYUDEBANTIAOJIXULENGZANGCHULI。FENBIESHEBIAOZHUN

火马电竞 PINZU(6 GENONGDU)、KONGBAIKONG、DAICEYANGPINZU。

火马电竞 ZHU:WEIJIANSHAOSHIYANWUCHA,BAOZHENGZHUNQUEXING,JIANYISHEZHIFUKONG。

火马电竞 3. JIAYANG:YIZHAOBIAOZHUNPINDESHUNXUFENBIEJIARU 100ul DEBIAOZHUNPINRONGYEYUKONGBAIWEIKONGZHONG;KONGBAIDUIZHAOKONGJIARU 100ul DEZHENGLIUSHUI;QIYUWEIKONGZHONGJIARU

100ul DEDAICEYANGBEN。

4. JIAMEIBIAORONGYE:BIAOZHUNPINZU、DAICEYANGBENZUGEKONGZHONGJIARU 50ul DEMEIBIAORONGYE(KONGBAIDUIZHAOKONGCHUWAI)。

火马电竞 5. WENYU:MEIBIAOBANYONGFENGBANZHIMIFENGHOU,FANGRUSHIHENEIYU 37℃HENGWENFUYU 1 XIAOSHI。

火马电竞 6. XIBAN:YONGXISHIHOUDEXIDIYEZHUMANMEIKONG,JINGZHI 15-30s,CHONGFENQINGXIMEIBIAOBAN 5 CI,YONGXISHUIZHICHEDIPAIGAN。

7. XIANSE:GEKONGJIARUXIANSEJI A YE 50ul HOU,ZAIJIARUXIANSEJI B YE 50ul。

8. ZHONGZHI:25-37℃XIABIGUANGFANYING 10-15 FENZHONG,JIARU 50ul ZHONGZHIYE。

9. DUBAN:ZAI 450nm BOZHANGDUQUGEKONGDE OD ZHI。

ZHUYISHIXIANG:

1. SHIYANCAOZUOZHONGBIXUSHIYONGYICIXINGXITOU,BIMIANJIAOCHAWURAN。

火马电竞 2. JIAYANG:JIAYANGSHI,YAOKONGZHIJIAYANGSUDU,BIMIANDIYIKONGYUZUIHOUYIKONGJIANDESHIJIANJIANGEGUODA,FOUZEJIANGHUIDAOZHIBUTONGDEYUFUYUSHIJIAN,CONGERYINGXIANG

火马电竞 SHIYANDEZHUNQUEXINGYIJIZHONGFUXING。

3. FUYU:YANGEANZHAOSHUOMINGSHUSHANGGUIDINGDEFUYUSHIJIANHEWENDUJINXING。

火马电竞 4. FANYINGSHIJIANDEKONGZHI:JIARUDIWUHOUQINGDINGSHIGUANCHAFANYINGKONGDEYANSEBIANHUA,RUGUOYANSEJIAOSHEN,QINGTIQIANJIARUZHONGZHIYEZHONGZHIFANYING。

火马电竞 5. JIANYISHIYANQIANYUCEYANGPINHANLIANG,RUYANGPINNONGDUGUOGAO,YINGDUIYANGPINJINXINGXISHI,JISUANJIEGUOSHICHENGYIXIANGYINGDEXISHIBEISHU。

6. JIANYISHIYONGBENSHIJIHESHIXIANZUOYUSHIYAN(JIXIANZUOBIAOZHUNQUXIAN,SHIYONGJIGEBIAOBEN),RUGUODUIBENSHIJIHEYOURENHEYIWEN,KEHESUOGOUJINGXIAOSHANGLIANXI,

火马电竞 RUGUOYINYUNSHUGUOCHENGDAOZHISHIJIHESHIXIAO,KEYAOQIUDIAOHUAN,DANGAIBUCHENGDANCHANPINBENSHENYIWAIDERENHESUNSHI。

ANQUANXING

1. BIMIANRENTIZHIJIEJIECHUXIANSEJIA、BHEZHONGZHIYE。YIDANJIECHUDAOZHEIXIEYETI,QINGJINKUAIYONGSHUICHONGXI。

2. SHIYANZHONGBUYAOJINSHI、CHOUYANHUOSHIYONGHUAZHUANGPIN。

3. BUYAOYONGZUIXIQUSHIJIHELIDERENHECHENGFEN。

大鼠核因子κB受体活化因子配基(RANKL)elisa试剂盒保存条件及有效期:

1.SHIJIHEBAOCUN:2-8℃

2.YOUXIAOQI:6GEYUE

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